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PubMed · 6581883

Simple method for preparing and quantifying very-low-density lipoprotein.

M Kohlmeier
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Abstract

A micromethod is described for isolating and quantifying human very-low-density lipoprotein (VLDL) from 0.1 mL of human serum, by which more than 100 samples can be processed in half a day with a standard ultracentrifuge. Replicate analysis of 10 normal and hyperlipemic samples gave median coefficients of variation of 11.7% for VLDL cholesterol, 5.1% for VLDL triglycerides. Results obtained by micro-ultracentrifugation and enzymic cholesterol measurement agreed closely with those measured with an electrophoretic method (Pearson's r = 0.98). Compared with the established method, on the other hand, lower values were found for both VLDL cholesterol (r = 0.89) and triglycerides (r = 0.97).

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CholesterolCholesterol, VLDLElectrophoresis, Agar GelHumansHyperlipidemiasLipoproteins, VLDLTriglyceridesJournal ArticleResearch Support, Non-U.S. Gov't

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M Kohlmeier. 1984. Simple method for preparing and quantifying very-low-density lipoprotein.. https://pubmed.ncbi.nlm.nih.gov/6581883/

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