PubMed · 6481466
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A S Eadie, T E Hilditch. 1984. Re: Improved intrinsic resolution: does it make a difference?. https://pubmed.ncbi.nlm.nih.gov/6481466/
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Many optical imaging systems have been designed to achieve spatial resolution exceeding the limit proposed by Abbe theory, namely lambda/2NA. These systems invariably use light inefficiently. We discuss the application of information theory to this problem, in an effort to find an upper limit to the theoretical efficiency of a super-resolving system, as a function of the degree to which its capabilities exceed the Abbe limit.
A two-pinhole axially superresolving confocal fluorescence imaging system is presented. Based on the concept of subtractive incoherent imaging, the system described here is equipped with a zero-focus complex-transmittance pupil filter in one of the collector paths. The optical sectioning capacity of the system is 25% superior to that of a free-pupil one-pinhole instrument.
Distortions in confocal 3D image data sets were related to movements of the microscope stage that matched fluctuations in laboratory temperature. Movement was apparent in all three axes. Adding a draught-proof enclosure, covering the stage, objective lenses and supporting structures, minimized these short-term fluctuations. However, the stage still tracked slow changes in laboratory temperature and was sensitive to heat sources mounted on the microscope. Suggestions are made about microscope design.