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PubMed · 6433515

Decalcification for electron microscopy with L-ascorbic acid.

M Wakita·M Kobayashi·T Shioi
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Abstract

L-Ascorbic acid decalcification was used for electron microscopy of mammalian tooth germs and bone after fixation in a glutaraldehyde-paraformaldehyde mixture. The recommended decalcifying solution is 2% with respect to L-ascorbic acid and 0.9% with respect to sodium chloride. The method has the advantage that decalcification is complete within a quarter of the time required with EDTA. The fine structure of ameloblasts and hard tissue is preserved as well as with EDTA.

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AmeloblastsAnimalsAscorbic AcidDecalcification TechniqueEdetic AcidJawMicroscopy, ElectronRatsTooth GermComparative StudyJournal Article

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M Wakita, M Kobayashi, T Shioi. 1983. Decalcification for electron microscopy with L-ascorbic acid.. https://doi.org/10.3109/10520298309066809

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