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PubMed · 6258146

A subcloning strategy for DNA sequence analysis.

A M Frischauf·H Garoff·H Lehrach
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Abstract

We describe here a new strategy of fragment preparation for sequencing procedures using endlabelled DNA fragments as substrates (2,3) which is directly applicable to DNA fragments cloned into the Pst I site of pBR322, or in modified form, to inserts into the BamH I or Sal I site of the same plasmid. Ordered sets of subclones of predetermined overlap are are generated. These can be sequenced directly without further strand- or fragment separation steps.

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Base SequenceCloning, MolecularDNA Restriction EnzymesDNA, BacterialDNA, RecombinantDNA, ViralEscherichia coliMethodsPlasmidsSemliki forest virusJournal Article

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A M Frischauf, H Garoff, H Lehrach. 1980-12-11. A subcloning strategy for DNA sequence analysis.. https://doi.org/10.1093/nar%2F8.23.5541

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