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PubMed · 6243500

Enzyme formation during solid-substrate fermentation in rotating vessels.

R W Silman
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Abstract

Aspergillus awamori NRRL 4869 was cultured on the solid substrate, wheat bran, in a modified Rollacell apparatus to produce alpha-galactosidase and invertase. The swivel cap on the elongated bottle permits the introduction of air while the bottle rotates. Parameters of air flow rate (0.05-0.2 liter/kg/min), rpm (0.15-15 rpm), and weight of solids (150 and 300 g) were varied. At low air flow rates (0.05 liter/kg solids/min), alpha-galactosidase production was minimal independent of the rotation rate. At 0.15 rpm and 0.2 liter/kg solids/min air flow rate, invertase production ceased after five days; whereas alpha-galactosidase production continued. The modified Rollacell can be a useful apparatus for studying solid-substrate cultures.

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R W Silman. 1980. Enzyme formation during solid-substrate fermentation in rotating vessels.. https://doi.org/10.1002/bit.260220213

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