[Decrease in the lipid content of human atherosclerotic aorta cells as affected by compounds increasing the intracellular level of cyclic AMP].

V V Tertov; A N Orekhov; V N Smirnov

Home/Archive/PubMed

PubMed · 6199053

[Decrease in the lipid content of human atherosclerotic aorta cells as affected by compounds increasing the intracellular level of cyclic AMP].

V V Tertov·A N Orekhov·V N Smirnov

View original source ↗Export metadata ↓

Abstract

A study was made of the effect of cholera toxin, an adenylate cyclase activator, and methylisobutylxanthine, an inhibitor of cAMP phosphodiesterase, on the content of main class lipids in intimal cells isolated from unaffected and atherosclerotic areas of the human aorta in primary culture. Both the agents did not change the content of lipids in the cells obtained from the normal intima. Cholera toxin and methylisobutylxanthine decreased the levels of cholesterol esters and triglycerides in the cells isolated from fatty layers and atherosclerotic patches. The agents under study did not produce any influence on the levels of phospholipids and free cholesterol in all cases investigated. It is assumed that compounds that increase intracellular cAMP may contribute to lipoidosis diminution in vivo.

Explore related subjects

1-Methyl-3-isobutylxanthine Aortic Diseases Arteriosclerosis Cholera Toxin Cholesterol Cholesterol Esters Cyclic AMP Humans In Vitro Techniques Lipid Metabolism Phospholipids Theophylline Triglycerides English Abstract Journal Article

Keep this discovery

Explore connections, maps & timelines

BibTeX RIS

V V Tertov, A N Orekhov, V N Smirnov. 1984. [Decrease in the lipid content of human atherosclerotic aorta cells as affected by compounds increasing the intracellular level of cyclic AMP].. https://pubmed.ncbi.nlm.nih.gov/6199053/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2006

Characterization of human islet-like structures generated from pancreatic precursor cells in culture.

This study addresses the characterization of human islet-like structures generated from a newly discovered sparse population of precursor cells (Petropavlovskaia and Rosenberg, 2002) in the human pancreas. These cells may be progenitor cells capable of producing pancreatic cells suitable for the treatment of type 1 diabetes. The cells were cultured successfully in non-adherent stationary cultures and yielded, as an important first step, a 1.9-fold expansion in a serum-free medium developed specifically for this cell type. This expanded population grew as pancreatic cell aggregates, which were analyzed for islet-like characteristics. Specifically, through RT-PCR analyses and functionality assays, we show that cells within the population expressed all four of the endocrine hormone genes and proteins (insulin, glucagon, somatostatin, pancreatic polypeptide). As well, the expanded pancreatic precursor cell population exhibited glucose responsiveness although the produced cells appeared to be still primitive in nature.

1-Methyl-3-isobutylxanthine↗

PubMed2006

Effect of aging on corticosterone secretion in diestrous rats.

The roles of age and prolactin (PRL) in regulating glucocorticoid secretion in diestrous rats were investigated. Adrenal zona fasciculata-reticularis (ZFR) cells from young, adult, middle (mid)-aged, and old female rats were isolated. Estrous cycle stage was determined by light microscopy after vaginal smears. Blood samples were collected from right jugular vein at 0, 30, 60, and 120 min after challenge with adrenocorticotropin (ACTH). During the diestrous phase, plasma levels of estradiol and progesterone were lower in mid-aged and old rats than in either young or adult rats. Age-dependent increases of the basal levels of plasma PRL and corticosterone were observed. No difference of ACTH-increased plasma concentrations of corticosterone was observed among young, adult, mid-aged, and old rats. Aging increased the basal, ACTH-, PRL-, forskolin (an adenylate cyclase activator)-, and 3-isobutyl-l-methylxanthine (IBMX, a non-selective phosphodiesterase inhibitor)-stimulated release of corticosterone and production of adenosine 3', 5'-cyclic monophosphate (cAMP) in ZFR cells. However, the 8-Br-cAMP (a membrane-permeable cAMP)-stimulated release of corticosterone was not affected by age. Taken together, these data indicated that aging increased corticosterone secretion in female rats during diestrous phase, which is in part due to an increase in cAMP accumulation. In conclusion, aging and PRL play a stimulatory role in the co-regulation of corticosterone secretion.

1-Methyl-3-isobutylxanthine↗

PubMed2005

Novel indole-based peroxisome proliferator-activated receptor agonists: design, SAR, structural biology, and biological activities.

The synthesis and structure-activity relationship studies of novel indole derivatives as peroxisome proliferator-activated receptor (PPAR) agonists are reported. Indole, a drug-like scaffold, was studied as a core skeleton for the acidic head part of PPAR agonists. The structural features (acidic head, substitution on indole, and linker) were optimized first, by keeping benzisoxazole as the tail part, based on binding and functional activity at PPARgamma protein. The variations in the tail part, by introducing various heteroaromatic ring systems, were then studied. In vitro evaluation led to identification of a novel series of indole compounds with a benzisoxazole tail as potent PPAR agonists with the lead compound 14 (BPR1H036) displaying an excellent pharmacokinetic profile in BALB/c mice and an efficacious glucose lowering activity in KKA(y) mice. Structural biology studies of 14 showed that the indole ring contributes strong hydrophobic interactions with PPARgamma and could be an important moiety for the binding to the protein.

1-Methyl-3-isobutylxanthine↗