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Three complementation groups in Cockayne syndrome.

A R Lehmann
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Abstract

After 16 Jm-2 of UV-irradiation non-dividing normal cells recover normal rates of RNA synthesis within 24 h, whereas in cells from donors with Cockayne syndrome (CS) the rate of RNA synthesis gradually declines. Cultures of a mixed population from 2 CS donors were fused with polyethylene glycol; subsequently they were UV-irradiated and RNA synthesis was measured autoradiographically in mono-, bi-, and multinuclear cells. Genetic complementation was indicated by high levels of RNA synthesis in bi- and multinuclear cells when compared with mononuclear cells. Using this assay, 11 CS strains have been assigned to three complementation groups: 2 into group A, 8 into group B and 1 into group C. The strain in group C is derived from an individual who also had xeroderma pigmentosum (XP), and was the sole known representative of XP-complementation group B.

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Cockayne SyndromeDwarfismGenetic Complementation TestHumansHybrid CellsRNAUltraviolet RaysXeroderma PigmentosumJournal ArticleResearch Support, Non-U.S. Gov't

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A R Lehmann. 1982. Three complementation groups in Cockayne syndrome.. https://doi.org/10.1016/0027-5107(82)90115-4

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