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PubMed · 591723

New method for obtaining IgA-specific protease.

T B Higerd·G Virella·R Cardenas·J Koistinen·J W Fett
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Abstract

A simple method for obtaining an active preparation of IgA-specific protease from a bacterial source is presented. In this method Streptococcus sanguis was inoculated onto the surface of a dialysis membrane on nutrient agar. Following growth, the membrane was removed from the agar surface and washed in a small volume of buffer. A solution with protease activity against IgA1 monoclonal proteins was obtained by clarification of the wash and appeared to be similar to enzyme preparations obtained by other methods.

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Antibody SpecificityElectrophoresis, Polyacrylamide GelImmunoelectrophoresisImmunoglobulin APeptide HydrolasesStreptococcus sanguisJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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T B Higerd, G Virella, R Cardenas, J Koistinen, J W Fett. 1977. New method for obtaining IgA-specific protease.. https://doi.org/10.1016/0022-1759(77)90178-8

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