PubMed · 5336792
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S Tolansky. 1963. Interferometry and biological microtopography.. https://doi.org/10.1016/0022-5193(63)90066-3
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We describe an original microscope for high-resolution optical coherence tomography applications. Our system is based on a Linnik interference microscope with high-numerical-aperture objectives. Lock-in detection of the interference signal is achieved in parallel on a CCD by use of a photoelastic birefringence modulator and full-field stroboscopic illumination with an infrared LED. Transverse cross-section (en-face, or XY) images can be obtained in real time with better than 1-microm axial (Z) resolution and 0.5-microm transverse (XY) resolution. A sensitivity of approximately 80 dB is reached at a 1-image/s acquisition rate, which allows tomography in scattering media such as biological tissues.
The interferometric measurement of abrupt surface steps in the micrometer range requires additional means to give unique results. It will be shown, that advantageous interpretation of the interferometric fringes are possible by combining a two-ray interferometer with a monochromator.
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