PubMed · 5268583
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1970. An improved Snyder Test.. https://pubmed.ncbi.nlm.nih.gov/5268583/
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When using quantitative light-induced fluorescence a number of factors can influence illumination level. The purpose of this study was to investigate, using a high-resolution camera and fibre-optic light source, the impact of illumination level and focal distance on common quantitative light-induced fluorescence outcomes. Twenty-four extracted teeth were examined using 6 illumination levels and 4 focal distances. Analysis was conducted using multiple linear regression models fitted to log DeltaQ, log DeltaF and log area with clustering of teeth and robust standard errors. Separate models were used for the different light and focal levels. The regression coefficients were significant for both DeltaQ and DeltaF but not area. Despite the significant regressions the actual effect was very small, and unlikely to confound clinical trial or practice results.
The purpose of this study was to determine whether quantitative light-induced fluorescence (QLF) could detect very early demineralization and remineralization longitudinally adjacent to orthodontic components in an in vitro model. Extracted human premolars (n = 13) were sectioned sagittally to produce two equal halves and an orthodontic cleat was bonded to the buccal surface of each tooth. Transparent nail varnish was placed over the remaining surface, leaving exposed enamel windows adjacent to the cleat on the coronal and gingival aspects. Each half-tooth was placed into the lid of an Eppendorf tube and randomly assigned to either control (distilled water) or experimental (lactic acid demineralizing buffer, pH 4.5) regimes. Digital photographs and QLF baseline images were taken. The tubes were mounted into a rotating holder and left for 24 hours. QLF and digital photographs were taken, the solutions refreshed and the teeth returned. This was continued every 48 hours for 288 hours. At this time the lactic acid buffer was replaced with a remineralizing solution (artificial saliva, fluoride, calcium) and the experiment continued with weekly examinations. QLF images were analysed and deltaQ at the 5 per cent threshold recorded. Analysis of the QLF images showed that both demineralization and remineralization were identified and monitored. Statistical differences between each of the timed examinations were found (P < 0.05). Analysis of the photographs demonstrated that QLF detected subclinical lesions. This initial pilot study has demonstrated the potential for QLF to longitudinally monitor de- and re-mineralization of enamel adjacent to orthodontic cleats in vitro.
The most commonly used clinical signs of root caries are visual (color, contour, surface cavitation) and tactile (surface texture) descriptions of a lesion. The traditional methods of visual-tactile diagnosis for root caries can produce a correct diagnosis but usually not until the lesion is at an advanced stage. Despite the subjectivity inherent in interpreting the clinical signs of root caries diagnosis, good to excellent inter-examiner reliability has been reported in clinical studies; however, the presence of filled surfaces dramatically enhances the agreement. When only untreated root caries is diagnosed, examiner reliability is reduced considerably. Clinicians look to diagnostic tests in the hope that they will perform better (that is, be more reliable) than clinical diagnosis and, therefore, can be used to replace clinical diagnosis. From the limited data available on diagnostic tests for root caries, tests determining the presence or absence of mutans streptococci and Lactobacilli are the most clinically helpful, producing calibrated efficiency scores exceeding 40 percent. The risk assessment approach to root caries diagnosis involves the determination of a patient's risk through the interpretation of clinical signs and the selection and application of an appropriate diagnostic test if the clinician is unsure of the diagnosis.