PubMed · 5249163
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1968. L-Forms.. https://pubmed.ncbi.nlm.nih.gov/5249163/
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No major differences have been found in series of Staphylococcus aureus strains which reverted from 'L'-phase, either by pyrolysis mass spectrometry or by phage-typing or sensitivity testing. In 'L'-phase they have been subcultured for a long time or transformed/reverted many times into/from 'L'-phase. Plasmids were lost during transformations/reversions, but there was some difference between the tetracycline-connected plasmids on the one hand and the erythromycin-connected ones on the other.
Stable L-forms of Salmonella typhi and Listeria monocytogenes were produced using penicillin (4500 units/ml) as inducer, and sucrose, normal horse serum and Mg++ as stabilizers. Stable L-forms were produced after 100 and 56 passages, then adapted to grow and multiply in a medium free of inducer and stabilizers so that they did not revert to parental forms even after 12 continuous passages.
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