PubMed · 5191425
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1969. [Digestive function test (Goiffon's technic)].. https://pubmed.ncbi.nlm.nih.gov/5191425/
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Fungal mycelial proteins are promising sustainable protein sources, yet their nutritional utilization is often limited by structural constraints. This study systematically evaluated the effects of protein isolation on the proteomic composition, gastrointestinal digestion behavior, amino acid utilization, and bioactivity of Pleurotus citrinopileatus mycelial proteins. Quantitative proteomics identified 3591 proteins, of which 3374 were shared between mycelial flour (PCMF) and protein isolate (PCMPI), indicating that PCMPI primarily represents the soluble proteome fraction. In vitro digestion revealed that PCMPI exhibited significantly higher digestibility (93.98%) than PCMF (42.98%) (p < 0.05), reaching levels comparable to whey protein isolate. Enhanced enzymatic accessibility in PCMPI promoted rapid peptide generation during the gastric phase and efficient amino acid release during the intestinal phase, resulting in higher peptide (634.76 mg/g) and free amino acid levels (341.69 mg/g) at the digestion endpoint. Consequently, PCMPI achieved a balanced amino acid profile with a PDCAAS of 1.0. Moreover, its digestion products exhibited stronger antioxidant activity (IC₅₀ = 8.36 mg/mL) and ACE inhibitory activity (IC₅₀ = 15.65 mg/mL) compared with PCMF. Mechanistically, protein isolation disrupted the cell wall matrix, shifting digestion from a structure-limited to an accessibility-driven regime. Collectively, these findings demonstrate that protein isolation markedly enhances the digestibility, nutritional quality, and functional potential of mycelial proteins, supporting their application as high-value sustainable protein ingredients.
Phytic acid, polyphenols, protein and starch digestibility (in vitro) in two varieties, namely CS-46 and CS-88, of soaked, dehulled and germinated cowpeas were determined. Soaking for 12 hours, dehulling of soaked seeds and germination for different time periods (24, 36 and 48 h) contributed significantly in reducing the phytic acid and polyphenol content of cowpeas. Removal of seed coat (dehulling) of soaked cowpeas reduced the polyphenols by 70-71%. Soaking (12 h) brought about an improvement in protein and starch digestibility which further increased after dehulling. Progressive increase in digestibility (protein and starch) was noticed, with an increase in germination period. Dehulling of soaked seeds was most effective in reducing the polyphenolic content, and germination in enhancing protein and starch digestibility.
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