PubMed · 490903
The source did not provide an abstract. Follow the original record for more information.
Explore connections, maps & timelines
N Hattori. 1979-06-29. [Recent progress in neoplastic cell function tests].. https://pubmed.ncbi.nlm.nih.gov/490903/
Cite the original work for its findings. Save a collection to share your selection of sources.
KEEP EXPLORING
The SUMO family in vertebrates includes at least three distinct proteins (SUMO-1, -2, and -3) that are added as post-translational modifications to target proteins. A considerable number of SUMO-1 target proteins have been identified, but little is known about SUMO-2. A stable HeLa cell line expressing His6-tagged SUMO-2 was established and used to label and purify novel endogenous SUMO-2 target proteins. Tagged forms of SUMO-2 were functional and localized predominantly in the nucleus. His6-tagged SUMO-2 conjugates were affinity-purified from nuclear fractions and identified by mass spectrometry. Eight novel potential SUMO-2 target proteins were identified by at least two peptides. Three of these proteins, SART1, heterogeneous nuclear ribonucleoprotein (RNP) M, and the U5 small nuclear RNP 200-kDa helicase, play a role in RNA metabolism. SART1 and heterogeneous nuclear RNP M were both shown to be genuine SUMO targets, confirming the validity of the approach.
Explore the source record for details and available documents.
Explore the source record for details and available documents.