Some improved methods in P22 transduction.

P E Hartman

doi:10.1093/genetics/76.4.625

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Some improved methods in P22 transduction.

P E Hartman

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Abstract

Recent refinements simplify methods for P22 transduction in Salmonella and allow improved recovery of phage-free transductional clones. The methods include use of: integration- and lysis-defective phage mutants, heat-killed bacteria to eliminate free phage, direct plating of phage and bacteria, replica-plating for detection of phage content of individual clones, improved broth for phage growth, and procurement of high titer phage from P22 lysogens.

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Clone Cells Culture Media Lysogeny Methods Mutation Radiation Genetics Recombination, Genetic Salmonella Phages Salmonella typhimurium Transduction, Genetic Ultraviolet Rays Journal Article

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P E Hartman. 1974. Some improved methods in P22 transduction.. https://doi.org/10.1093/genetics%2F76.4.625

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