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PubMed · 42699722

Identification of elements determining KIR gene demethylation at the CD56-bright stage of NK cell development.

Hongchuan Li·Noah T Koenigs·Michael Ruesch·Ella Troy·Olivia Brancato-Buentello·Zhonghe Sun·Xiaolin Wu·Xiongfong Chen·Yongmei Zhao·Aharon G Freud·Stephen K Anderson
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Abstract

The variegated expression of the KIR family of class I MHC receptors generates specialized natural killer (NK) cells capable of allele-specific HLA recognition. Understanding the mechanism of KIR gene activation will lead to improved methods for the generation of fully functional NK cells. A central RUNX-binding site in the KIR proximal promoter is required for gene activation. RUNX proteins recruit ten-eleven translocation (TET) proteins that generate 5-hydroxymethylcytosine (5hmC) and drive DNA demethylation. Assessment of 5-methylcytosine (5mC) and 5hmC residues at four stages of NK cell development reveals deposition of 5hmC primarily in a CREB site next to the RUNX site at the CD56Bright stage but not the subsequent CD56Dim stage representing fully mature NK cells. KIR promoter demethylation is delayed relative to other lineage-associated genes, indicating a high threshold for KIR gene demethylation in developing NK cells, and a window of opportunity for RUNX/TET-dependent KIR gene activation in CD56Bright NK cells.

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6-base sequencingKIRNK cellsTET2epigenetic regulationhumanhydroxymethylcytosineJournal Article

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Hongchuan Li, Noah T Koenigs, Michael Ruesch, Ella Troy, Olivia Brancato-Buentello, Zhonghe Sun, Xiaolin Wu, Xiongfong Chen, Yongmei Zhao, Aharon G Freud, Stephen K Anderson. 2026-08-26. Identification of elements determining KIR gene demethylation at the CD56-bright stage of NK cell development.. https://doi.org/10.1016/j.isci.2026.117350

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