Applications of transposon-insertion sequencing for understanding bacterial physiology.

Emily C A Goodall; Stineke van Houte

doi:10.1099/mgen.0.001796

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PubMed · 42690707

Applications of transposon-insertion sequencing for understanding bacterial physiology.

Emily C A Goodall·Stineke van Houte

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Abstract

Transposon-insertion sequencing (Tn-seq) couples transposon mutagenesis with next-generation sequencing to identify the transposon insertion site for thousands of mutants in parallel. It is a powerful technology with a myriad of uses beyond the identification of essential genes required for a cell to grow and divide. Tn-seq is particularly useful as a high-throughput method to assign function to function-unknown genes, which have increased steadily with the abundance of newly sequenced bacterial genomes. Tn-seq has now been adapted for use in over 100 bacterial species. Here, we summarize the applications of Tn-seq for querying bacterial physiology and discuss some of the possible applications for the future.

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DNA Transposable Elements Mutagenesis, Insertional High-Throughput Nucleotide Sequencing Bacteria Genome, Bacterial Bacterial Physiological Phenomena Sequence Analysis, DNA INSeq TraDIS functional genomics transposon mutagenesis transposon-insertion sequencing (Tn-seq)Journal Article Review

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Emily C A Goodall, Stineke van Houte. 2026. Applications of transposon-insertion sequencing for understanding bacterial physiology.. https://doi.org/10.1099/mgen.0.001796

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