Detoxifying biotransformation of chloramphenicol by Exiguobacterium sp. CAP4 and its bioaugmentation of chloramphenicol biodegradation in simulated wastewater.

Shiyu Yuan; Jingyao Ping; Huayi Chen; Jie Liu; Zewen Tan

doi:10.1016/j.jes.2025.10.050

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Detoxifying biotransformation of chloramphenicol by Exiguobacterium sp. CAP4 and its bioaugmentation of chloramphenicol biodegradation in simulated wastewater.

Shiyu Yuan·Jingyao Ping·Huayi Chen·Jie Liu·Zewen Tan

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Abstract

The extensive use of chloramphenicol (CAP) in livestock leads the accumulation of CAP in livestock manures, threatening environmental and human health. Therefore, eliminating or reducing CAP concentration in manures before its re-utilization and application through microbial remediation is necessary. Exiguobacterium sp. CAP4, isolated from the plastisphere in duck manures, was capable of degrading CAP with the biodegradation efficiency of 97.8 % at initial CAP concentration of 5 mg/L within 4 days. A total of twenty-four biotransformation products were determined, including two novel transformation products, TP166 and TP203, enriched the integrity of CAP biodegradation pathways. Furthermore, the biotransformation process was proposed as a detoxifying process through biotransformation products toxicity evaluation. Notably, Exiguobacterium sp. CAP4 successfully colonized in the cow manures after inoculation, and bioaugmented the biodegradation of CAP in virgin cow manures. This study significantly extended our understanding of the CAP biotransformation fate, and provided a promising bacterial strain for bioremediation of CAP containing wastewater in situ.

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Chloramphenicol Biodegradation, Environmental Biotransformation Wastewater Water Pollutants, Chemical Exiguobacterium Waste Disposal, Fluid Anti-Bacterial Agents Animals Bioaugmentation Biotransformation pathway Exiguobacterium sp. CAP4 Toxicity assessment Journal Article

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Shiyu Yuan, Jingyao Ping, Huayi Chen, Jie Liu, Zewen Tan. 2025-10-30. Detoxifying biotransformation of chloramphenicol by Exiguobacterium sp. CAP4 and its bioaugmentation of chloramphenicol biodegradation in simulated wastewater.. https://doi.org/10.1016/j.jes.2025.10.050

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