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MMP-2 rs243865 Polymorphism as a Risk Modifier of Hepatocellular Carcinoma in Taiwanese Males, Smokers and Alcohol Consumers.

Te-Cheng Yueh·Ming-Hsien Wu·Mei-Due Yang·Meng-Chun Lu·Yen-Chen Shao·Tao-Wei Ke·Zhi-Hong Wang·Chi-An Chen·Chia-Wen Tsai·DA-Tian Bau·Wen-Shin Chang
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Abstract

BACKGROUND/AIM: Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related mortality worldwide. Matrix metalloproteinase-2 (MMP-2) plays an important role in extracellular matrix remodeling and HCC progression. This study investigated the associations of two functional MMP-2 promoter polymorphisms, rs243865 and rs2285053, with HCC susceptibility in a Taiwanese population and explored their potential interactions with environmental risk factors. MATERIALS AND METHODS: A hospital-based case-control study was conducted at China Medical University Hospital (Taichung, Taiwan), involving 298 HCC patients and 889 age- and sex-matched cancer-free controls recruited in Taiwan. MMP-2 rs243865 and rs2285053 genotypes were determined utilizing polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods. Stratified analyses were used to explore their potential interactions between MMP-2 genotypes and environmental factors including sex, smoking and alcohol drinking status. RESULTS: No significant association was observed between HCC susceptibility and either rs243865 (CT+TT versus CC: odds ratio (OR)=1.20, 95% confidence interval (CI)=0.87-1.66, p=0.2944] or rs2285053 (CT+TT versus CC: OR=1.12, 95% CI=0.86-1.46, p=0.4219). Similarly, allelic analyses revealed no significant effects. Interestingly, stratified analyses demonstrated significant gene-environment interactions for rs243865. Male carriers of the TT genotype exhibited an increased HCC risk (OR=3.24, 95% CI=1.12-9.37, p=0.0488). Among smokers, the TT genotype was associated with a markedly elevated risk (OR=6.46, 95% CI=1.65-25.29, p=0.0055), while alcohol drinkers carrying the TT genotype showed the highest susceptibility (OR=9.69, 95% CI=1.99-47.13, p=0.0022). No significant association was found for rs2285053 genotype in any genetic variant and subgroup. CONCLUSION: Although MMP-2 rs243865 and rs2285053 do not independently determine HCC susceptibility, rs243865 T allele may serve as a genetic biomarker for identifying Taiwanese males, smokers, and alcohol drinkers at elevated risk of HCC. These findings highlight the importance of gene-environment interactions in hepatocarcinogenesis and support the incorporation of genetic information into personalized HCC risk assessment and prediction strategies.

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HumansMaleMatrix Metalloproteinase 2Carcinoma, HepatocellularLiver NeoplasmsAlcohol DrinkingGenetic Predisposition to DiseaseMiddle AgedCase-Control StudiesPolymorphism, Single NucleotideSmokingTaiwanRisk FactorsGenotypeFemaleAgedPromoter Regions, GeneticGene-Environment InteractionAsian PeopleAgegenotypehepatocellular carcinomamatrix metalloproteinase-2polymorphismsexJournal Article

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Te-Cheng Yueh, Ming-Hsien Wu, Mei-Due Yang, Meng-Chun Lu, Yen-Chen Shao, Tao-Wei Ke, Zhi-Hong Wang, Chi-An Chen, Chia-Wen Tsai, DA-Tian Bau, Wen-Shin Chang. 2026. MMP-2 rs243865 Polymorphism as a Risk Modifier of Hepatocellular Carcinoma in Taiwanese Males, Smokers and Alcohol Consumers.. https://doi.org/10.21873/anticanres.18339

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