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PubMed · 42645592

Synthetic transcriptional repression systems in plants.

Lindsey A Clark·Alexander C Pfotenhauer·Scott C Lenaghan·C Neal Stewart
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Abstract

Transcriptional repression is a fundamental regulatory mechanism that enables precise control of gene expression in response to developmental signals and environmental stimuli. Synthetic biology can leverage this process within plants to engineer programmable transgene repression systems. This review examines strategies for harnessing prokaryotic repressors in eukaryotic systems to develop synthetic repression systems in plants. These systems utilize modular promoter and repressor architectures that can be tuned through operator placement and repression-domain fusion, respectively, to adjust transcriptional regulation. Chemically dependent inducibility can also be introduced either through use of native derepression mechanisms of the prokaryotic repressors or the incorporation of ligand-binding domains. Finally, this review explores key challenges in designing synthetic repression systems, including kinetics constraints, balancing ON and OFF states, and differences between transient and transgenic expression contexts. Overall, this review highlights modular design frameworks for tunable transgene expression in plants.

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Gene Expression Regulation, PlantPromoter Regions, GeneticSynthetic BiologyPlantsPlants, Genetically ModifiedTranscription, GeneticTranscription FactorsRepressor ProteinsTransgenesRepression domainsSynthetic biologySynthetic promotersSynthetic transcription factorsTranscriptional regulationJournal ArticleReview

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Lindsey A Clark, Alexander C Pfotenhauer, Scott C Lenaghan, C Neal Stewart. 2026-08-26. Synthetic transcriptional repression systems in plants.. https://doi.org/10.1007/s00299-026-03949-9

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Gene Expression Regulation, Plant↗