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PubMed · 42632016

Emergent 3D genome reorganization and graded gene control from the stepwise assembly of transcriptional condensates.

Surabhi Chowdhary·Sarah Paracha·Lucas Dyer·David Pincus
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Abstract

Transcriptional condensates are clusters of transcription factors, coactivators, and RNA Pol II associated with gene activation, yet how they assemble and function within the cell remains unclear. Here, we show that transcriptional condensates form in a stepwise manner to enable both graded and three-dimensional (3D) gene control in the yeast heat shock response (HSR). First, the transcription factor Hsf1 (heat shock factor 1) clusters upon partial dissociation from the chaperone Hsp70. Next, the coactivator Mediator partitions following further Hsp70 dissociation and Hsf1 phosphorylation. Finally, Pol II condenses, driving emergent coalescence of HSR genes. Separation-of-function Hsf1 mutants revealed graded (non-switch-like) control of transcription and a decoupling of condensate formation and gene activation. Fully assembled HSR condensates promoted adaptive 3D genome reconfiguration, suggesting a role beyond transcription. In the HSR, differential condensation of the transcriptional machinery quantitatively tunes gene expression and qualitatively remodels the 3D genome.

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3D genomeCP: molecular biologyHsf1Hsp70MediatorRNA Pol IIbiomolecular condensateschaperonegene transcriptionheat shock responseproteostasisJournal Article

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Surabhi Chowdhary, Sarah Paracha, Lucas Dyer, David Pincus. 2026-08-22. Emergent 3D genome reorganization and graded gene control from the stepwise assembly of transcriptional condensates.. https://doi.org/10.1016/j.celrep.2026.117888

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