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Integrated transcriptomic and metabolomic analysis of fluoride tolerance-related pathways and differentially expressed genes in silkworm strain XSKD.

Xiaomeng Wei·Zhiyong Qiu·Dongxu Shen·Xuelian Zhang·Heying Qian·Dingguo Xia·Qiaoling Zhao
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Abstract

XueSong KD (XSKD) silkworm strain exhibits prominent fluoride tolerance, yet the underlying molecular mechanisms of fluoride tolerance remains unclear. In the present study, fourth-instar pre-molting XSKD silkworms were used as experimental materials for integrated transcriptomic and untargeted metabolomic analyses. In total, 572 differentially expressed genes and 90 differential metabolites were screened. GO enrichment and KEGG enrichment based on the hypergeometric distribution model revealed that 13-Hydroxy-9Z,11E-octadecadienoic acid (13-(S)-HODE) acts as the core differential metabolite, which is significantly enriched in the linoleic acid metabolism pathway. Within this pathway, LOC101737302 and CYP338A1 display opposite expression trends and show correlations with pathway metabolites. Based on multi-omics data, this study preliminarily characterizes the lipid metabolic response under fluoride stress, providing omics dataset support for further in-depth exploration of the molecular mechanism of fluoride tolerance in silkworms.

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AnimalsBombyxFluoridesTranscriptomeMetabolomicsMetabolomeGene Expression ProfilingMultiomicsInsect ProteinsBombyx moriCYP338A1Fluoride tolerancePhospholipaseJournal Article

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Xiaomeng Wei, Zhiyong Qiu, Dongxu Shen, Xuelian Zhang, Heying Qian, Dingguo Xia, Qiaoling Zhao. 2026-08-15. Integrated transcriptomic and metabolomic analysis of fluoride tolerance-related pathways and differentially expressed genes in silkworm strain XSKD.. https://doi.org/10.1016/j.cbd.2026.101973

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Journal Article resourceAnimals subjectBombyx subjectBombyx mori subjectCYP338A1 subjectFluoride tolerance subjectFluorides subjectGene Expression Profiling subjectInsect Proteins subjectJournal Article subjectMetabolome subjectMetabolomics subjectMultiomics subject

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