Single-cell RNA sequencing provides further insights into the immunostimulatory action of freeze-dried Lactiplantibacillus plantarum on Penaeus vannamei shrimp.

John Paul Matthew Domingo Guzman; Hiroki Kusaka; Claudio Gargano; Ma Ramela Angela Bermeo-Capunong; Mikio Aoki; Hiroshi Kuwahara; Hidehiro Kondo; Keiichiro Koiwai; Ikuo Hirono

doi:10.1016/j.fsi.2026.111643

PubMed · 42580374

Single-cell RNA sequencing provides further insights into the immunostimulatory action of freeze-dried Lactiplantibacillus plantarum on Penaeus vannamei shrimp.

John Paul Matthew Domingo Guzman·Hiroki Kusaka·Claudio Gargano·Ma Ramela Angela Bermeo-Capunong·Mikio Aoki·Hiroshi Kuwahara·Hidehiro Kondo·Keiichiro Koiwai·Ikuo Hirono

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Abstract

Immunostimulation through dietary interventions opened new avenues in developing disease control and prevention tools for shrimp aquaculture. We have previously shown that feeding with freeze-dried Lactiplantibacillus plantarum (LAB) increased disease resistance of Penaeus vannamei against both Vibrio parahaemolyticus and white spot syndrome virus (WSSV) based on bulk RNA sequencing of shrimp gills. This tissue participates in ion transport and serves as a first line of defense against environmental stressors and pathogenic infections. However, characterization of their cell composition and functions remains limited. Here, we implemented a single-cell RNA sequencing approach to further gather insights into how feeding with freeze-dried LAB modulates host immunity which may not be evident with bulk RNA sequencing approach. A total of five clusters with unique transcriptional signatures were identified, corresponding to pillar cells, septal cells, and sessile hemocytes. Pseudo-bulk analyses at global- and cluster-levels showed differential expression of genes related to host immunity and metabolism. We further revealed how overall transcriptomic changes are not exclusively caused by gene expression changes but may also be driven by cell population dynamics. This study highlighted how single-cell RNA sequencing approach may shed light on the mechanisms of action of immunostimulants which may be masked in bulk transcriptome analyses.

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Animals Penaeidae Lactiplantibacillus plantarum Sequence Analysis, RNA Immunity, Innate Freeze Drying Single-Cell Analysis Vibrio parahaemolyticus Transcriptome Probiotics White spot syndrome virus 1 Cell population dynamics Drop-seq Feed supplement Lactic acid bacteria Journal Article

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John Paul Matthew Domingo Guzman, Hiroki Kusaka, Claudio Gargano, Ma Ramela Angela Bermeo-Capunong, Mikio Aoki, Hiroshi Kuwahara, Hidehiro Kondo, Keiichiro Koiwai, Ikuo Hirono. 2026-08-11. Single-cell RNA sequencing provides further insights into the immunostimulatory action of freeze-dried Lactiplantibacillus plantarum on Penaeus vannamei shrimp.. https://doi.org/10.1016/j.fsi.2026.111643

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Journal Article resource Animals subject Immunity, Innate subject Journal Article subject Lactiplantibacillus plantarum subject Penaeidae subject Single-cell RNA sequencing method

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