Comparative assessment of post-transport disease susceptibility in Asian seabass (Lates calcarifer): Associations with oxidative stress, immune responses, gut microbiota, and tissue pathology.

Yosapon Adisornprasert; Pakapon Meachasompop; Benchawan Kumwan; Chonlatat Rajitdumrong; Pimrawee Chaemlek; Prapansak Srisapoome; Jitraporn Phaksopa; Passakorn Kingwascharapong; Channarong Rodkhum; Patcharapong Thangsunan; Pattanapong Thangsunan; Kanokporn Saenphet; Supap Saenphet; Putita Chokmangmeepisarn; Wararut Buncharoen; Anurak Uchuwittayakul

doi:10.1016/j.cbd.2026.101961

PubMed · 42574774

Comparative assessment of post-transport disease susceptibility in Asian seabass (Lates calcarifer): Associations with oxidative stress, immune responses, gut microbiota, and tissue pathology.

Yosapon Adisornprasert·Pakapon Meachasompop·Benchawan Kumwan·Chonlatat Rajitdumrong·Pimrawee Chaemlek·Prapansak Srisapoome·Jitraporn Phaksopa·Passakorn Kingwascharapong·Channarong Rodkhum·Patcharapong Thangsunan·Pattanapong Thangsunan·Kanokporn Saenphet·Supap Saenphet·Putita Chokmangmeepisarn·Wararut Buncharoen·Anurak Uchuwittayakul

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Abstract

Stress is a crucial factor that affects aquaculture systems, particularly during transportation, which often leads to deteriorated fish health and reduced survival rates. This study aimed to investigate the comparative differences in physiological changes, oxidative stress parameters, and immune responses between clinically healthy and diseased Asian seabass (Lates calcarifer) following commercial transportation. The study compared the health status of fish after transportation, categorized into healthy (Healthy) and diseased (Disease) groups. Assessments were conducted on oxidative stress parameters, immune responses, gut microbiota composition, and tissue pathology. The results showed that diseased fish exhibited significantly higher oxidative stress levels (P < 0.05), as indicated by an increase in malondialdehyde (MDA) levels and altered antioxidant and redox-related markers, including superoxide dismutase (SOD), nitric oxide (NO), catalase (CAT), glutathione (GSH), glutathione reductase (GR), and glutathione peroxidase (GPx), measured across multiple target tissues (head kidney, gills, liver, intestine, and brain), compared with healthy fish. Furthermore, the expression of immune-related genes was significantly downregulated in diseased fish after transportation, indicating immune suppression. In contrast, healthy fish maintained a more balanced immune response, which may partially mitigate the adverse effects of transport-induced stress. Gut microbiota analysis revealed that diseased fish had a significant reduction in beneficial bacteria such as Cetobacterium somerae and Bacillus spp., accompanied by a significant (P < 0.05) increase in opportunistic pathogens including Aeromonas spp., Photobacterium spp., and Vibrio spp. Histopathological examination showed severe damage in the gills, liver, and intestines of diseased fish (P < 0.05), while only minor tissue alterations were observed in healthy fish. Overall, the findings indicate that post-transport diseased Asian seabass exhibit marked oxidative stress, impaired antioxidant defense, altered immune responses, gut microbial dysbiosis, and multi-organ tissue damage compared with clinically healthy post-transport fish. These results suggest that deterioration of transport conditions may contribute to post-transport morbidity and disease susceptibility.

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Animals Oxidative Stress Gastrointestinal Microbiome Fish Diseases Disease Susceptibility Bass Transportation Antioxidant Asian seabass Gut microbiota Histopathology Immune response Lates calcarifer Oxidative stress Transportation stress Journal Article Comparative Study

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Yosapon Adisornprasert, Pakapon Meachasompop, Benchawan Kumwan, Chonlatat Rajitdumrong, Pimrawee Chaemlek, Prapansak Srisapoome, Jitraporn Phaksopa, Passakorn Kingwascharapong, Channarong Rodkhum, Patcharapong Thangsunan, Pattanapong Thangsunan, Kanokporn Saenphet, Supap Saenphet, Putita Chokmangmeepisarn, Wararut Buncharoen, Anurak Uchuwittayakul. 2026-07-31. Comparative assessment of post-transport disease susceptibility in Asian seabass (Lates calcarifer): Associations with oxidative stress, immune responses, gut microbiota, and tissue pathology.. https://doi.org/10.1016/j.cbd.2026.101961

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Journal Article; Comparative Study resource Animals subject Antioxidant subject Asian seabass subject Bass subject Comparative Study subject Disease Susceptibility subject Fish Diseases subject Gastrointestinal Microbiome subject Gut microbiota subject Histopathology subject Immune response subject Journal Article subject Lates calcarifer subject Oxidative Stress subject

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