Systematic identification pepper CaE2F transcription factor reveals the role of CaDPb in drought stress response.

Rong Lu; Yishui Chen; Longxue Li; Zilin Zhang; Qinghong Zhou; Meixia Wang; Huibin Han

doi:10.1016/j.plantsci.2026.113371

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Systematic identification pepper CaE2F transcription factor reveals the role of CaDPb in drought stress response.

Rong Lu·Yishui Chen·Longxue Li·Zilin Zhang·Qinghong Zhou·Meixia Wang·Huibin Han

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Abstract

The EARLY 2 FACTOR (E2F) transcription factor (TF) family plays a pivotal role in regulating plant development and adaptations to environmental stresses. However, the physiological function of E2Fs in pepper (Capsicum annuum L.) are not well elucidated. In this work, we conduct a comprehensive genome-wide annotation of the E2F family within the Zunla-1 pepper genome and further explore the biological roles of CaDPb in response to drought stress. Through systematic bioinformatics analysis, we identify a total of nine CaE2F genes within the Zunla-1 genome, categorizing them into three distinct subgroups. Additionally, we discover multiple cis-regulatory elements in the CaE2F promoter regions associated with responses to plant hormones and drought stress. Public RNA-seq datasets reveal distinct expression profiles of CaE2F genes across various pepper tissues and their responses to environmental stimuli and plant hormones. Subsequently, the CaDPb gene is further functionally verified in drought response. Our findings indicate that TRV2:CaDPb silenced pepper plants are more sensitivity to drought. Furthermore, we show that CaDPb participates in the regulation of reactive oxygen species (ROS) production, the expression of drought-responsive genes, and the modulation of stomatal aperture. Taken together, our findings provide a comprehensive characterization of E2F genes in pepper and offer insights into the biological function of CaDPb in pepper drought stress response.

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Capsicum Plant Proteins Drought Resistance Gene Expression Regulation, Plant Transcription Factors Reactive Oxygen Species Stress, Physiological Droughts E2F Transcription Factors Capsicum annuum L.Drought E2F transcription factor ROS Stomatal aperture Journal Article

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Rong Lu, Yishui Chen, Longxue Li, Zilin Zhang, Qinghong Zhou, Meixia Wang, Huibin Han. 2026-08-11. Systematic identification pepper CaE2F transcription factor reveals the role of CaDPb in drought stress response.. https://doi.org/10.1016/j.plantsci.2026.113371

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