Dynamic transcriptomic landscape from bulk RNA-seq reveals critical mmu-miR-181a-5p/hif1a and mmu-miR-101a-3p/col1a1 modules for deep second-degree burn wound healing.

Luofeng Jiang; Chuting Yu; Heng He; Weishi Kong; Jianyu Lu; Wei Zhang; Jiezhi Lin; Lu Yang; Tianyi Liu; Yongjun Zheng; Zhaofan Xia

doi:10.1016/j.ygeno.2026.111304

PubMed · 42567425

Dynamic transcriptomic landscape from bulk RNA-seq reveals critical mmu-miR-181a-5p/hif1a and mmu-miR-101a-3p/col1a1 modules for deep second-degree burn wound healing.

Luofeng Jiang·Chuting Yu·Heng He·Weishi Kong·Jianyu Lu·Wei Zhang·Jiezhi Lin·Lu Yang·Tianyi Liu·Yongjun Zheng·Zhaofan Xia

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Abstract

Burn injuries constitute a significant global health challenge, with deep partial-thickness burns (deep second-degree) posing particular clinical concerns due to prolonged healing and high scarring risks stemming from reticular dermis damage. Current therapeutic strategies remain largely empirical, reflecting limited understanding of stage-specific regulatory mechanisms. This study systematically investigated the molecular basis of deep partial-thickness burn repair by establishing murine models and performing RNA-seq analysis across healing phases (0, 3, 7, 14 days post-burn, dpb). Integrated bioinformatics revealed pivotal ceRNA and PPI networks, identifying hif1a (hypoxia-responsive immunomodulator) and col1a1 (ECM remodeling hub) as nodal regulators. Mechanistically, mmu-miR-101a-3p and mmu-miR-181a-5p were validated as post-transcriptional repressors of col1a1 and hif1a, respectively. Our work pioneers the discovery of the mmu-miR-181a-5p/hif1a and mmu-miR-101a-3p/col1a1 axes as master regulators of burn repair, offering novel therapeutic targets. The multi-omics dataset and molecular networks established herein provide a foundational resource for wound healing research.

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MicroRNAs Animals Burns Wound Healing Mice Collagen Type I Hypoxia-Inducible Factor 1, alpha Subunit Collagen Type I, alpha 1 Chain Transcriptome Humans Deep second-degree burns Wound healing mmu-miR-101a-3p/col1a1 axis mmu-miR-181a-5p/hif1a Journal Article

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Luofeng Jiang, Chuting Yu, Heng He, Weishi Kong, Jianyu Lu, Wei Zhang, Jiezhi Lin, Lu Yang, Tianyi Liu, Yongjun Zheng, Zhaofan Xia. 2026-08-07. Dynamic transcriptomic landscape from bulk RNA-seq reveals critical mmu-miR-181a-5p/hif1a and mmu-miR-101a-3p/col1a1 modules for deep second-degree burn wound healing.. https://doi.org/10.1016/j.ygeno.2026.111304

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