Antagonistic regulation by mango MiSPL9a and MiSPL9b regulates flowering time, drought and salt stress in Arabidopsis.

Yanshu Meng; Xiuxia He; Siyuan He; Kaijiang Li; Yuexing Zhang; Weiqiaochu He; Xinhua He; Cong Luo

doi:10.1016/j.gene.2026.150348

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Antagonistic regulation by mango MiSPL9a and MiSPL9b regulates flowering time, drought and salt stress in Arabidopsis.

Yanshu Meng·Xiuxia He·Siyuan He·Kaijiang Li·Yuexing Zhang·Weiqiaochu He·Xinhua He·Cong Luo

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Abstract

SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors, which are unique to plants, contain a highly conserved SBP domain that regulates gene expression by binding to downstream targets. They play critical roles in various biological processes, especially in the regulation of flowering in plants. In this study, two SPL-like genes (MiSPL9a and MiSPL9b) were identified from mango genomic and transcriptomic data, and their sequence, expression and function were further analyzed. Sequence analysis revealed that MiSPL9a and MiSPL9b have open reading frames of 1173 bp and 1158 bp, respectively, with slight differences in the number of cis-regulatory elements within their promoter regions. Expression analysis under stress conditions revealed distinct patterns: MiSPL9a expression significantly differed under drought stress but did not significantly differ under salt stress, whereas MiSPL9b expression responded significantly to salt stress but changed minimally under drought stress. Phenotypic analysis of the transgenic Arabidopsis lines revealed that MiSPL9a overexpression delayed flowering, whereas MiSPL9b overexpression promoted early flowering. Under stress conditions, compared with wild-type plants, MiSPL9a-overexpressing plants presented increased drought tolerance but did not significantly differ. In contrast, MiSPL9b-overexpressing plants were sensitive to salt stress, with no notable phenotypic differences observed under drought conditions. Physiological assays revealed that under drought stress, MiSPL9a transgenic plants presented significantly reduced levels of malondialdehyde (MDA) and hydrogen peroxide (H2O2) and increased proline (Pro) content and superoxide dismutase (SOD) activity. Under salt stress, MiSPL9b transgenic plants presented opposite trends in terms of these physiological markers. In summary, both MiSPL9a and MiSPL9b are involved in the regulation of plant flowering time and stress responses, but their functions differ.

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Arabidopsis Flowers Gene Expression Regulation, Plant Plants, Genetically Modified Plant Proteins Mangifera Transcription Factors Salt Stress Droughts Drought Resistance Stress, Physiological Promoter Regions, Genetic Abiotic stress Flowering regulation Gene function Mango MiSPL9a/9b Journal Article

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Yanshu Meng, Xiuxia He, Siyuan He, Kaijiang Li, Yuexing Zhang, Weiqiaochu He, Xinhua He, Cong Luo. 2026-08-05. Antagonistic regulation by mango MiSPL9a and MiSPL9b regulates flowering time, drought and salt stress in Arabidopsis.. https://doi.org/10.1016/j.gene.2026.150348

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Journal Article resource Journal Article subject Mangifera subject Mango subject Plant Proteins subject Plants, Genetically Modified subject Promoter Regions, Genetic subject

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