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Generation of FCGR3A-EGFP knock-in reporter human embryonic stem cell line, WAe001-A-3S, using CRISPR/Cas9n-based gene targeting.

Qifei Dong·Shahang Luo·Jiaqi Sun·Huisheng Liu
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Abstract

Fc gamma receptor IIIA (FCGR3A) encodes CD16a, a key mediator of antibody-dependent cellular cytotoxicity (ADCC) that regulates innate and adaptive immunity, especially in natural killer (NK) cells and monocytes. We generated an FCGR3A-EGFP knock-in human embryonic stem cell (hESC) line via CRISPR/Casn9n. The cell line showed a normal karyotype, maintained expression ofthe pluripotency markers OCT4, SOX2, and NANOG, and retained trilineage differentiation potential. This reporter line enables real-time tracking of FCGR3A expression during immune cell differentiation, serving as a useful tool for studying FCGR3A+ immune cell development and related immune mechanisms.

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HumansReceptors, IgGGreen Fluorescent ProteinsCRISPR-Cas SystemsHuman Embryonic Stem CellsGene Knock-In TechniquesCell DifferentiationCell LineGene TargetingGenes, ReporterJournal Article

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Qifei Dong, Shahang Luo, Jiaqi Sun, Huisheng Liu. 2026-07-15. Generation of FCGR3A-EGFP knock-in reporter human embryonic stem cell line, WAe001-A-3S, using CRISPR/Cas9n-based gene targeting.. https://doi.org/10.1016/j.scr.2026.104061

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