Lipid metabolism is a key central, systemic and gut microbial feature of the decline in rat hippocampal function during middle age.

Maria Giovanna Caruso; Sebastian Dohm-Hansen; Francisco Donoso; Zo&#xeb; A P Williams; Stefanie Grabrucker; Aonghus Lavelle; Jane A English; Sarah Nicolas; Olivia F O'Leary; Yvonne M Nolan

doi:10.1016/j.neurobiolaging.2026.07.001

PubMed · 42447757

Lipid metabolism is a key central, systemic and gut microbial feature of the decline in rat hippocampal function during middle age.

Maria Giovanna Caruso·Sebastian Dohm-Hansen·Francisco Donoso·Zoë A P Williams·Stefanie Grabrucker·Aonghus Lavelle·Jane A English·Sarah Nicolas·Olivia F O'Leary·Yvonne M Nolan

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Abstract

Middle age is emerging as a turning point in brain ageing, prognostic of future cognitive health and amenable to intervention. Metabolic and proteomic differences during this period are not yet fully understood and may potentially influence functions of the hippocampus, a brain area that regulates memory and anxiety. While the gut microbiota is implicated in brain ageing, the relationship between the gut microbiota, the metabolic state, and hippocampal proteome in middle age has not been investigated. We hypothesise that peripheral metabolic or protein features are associated with hippocampal vulnerability in middle age. Therefore, young adult and middle-aged rats were assessed for behavioural, proteomic, metabolic, and gut microbiota differences. Proteomic profiling of the hippocampus revealed differential expression of proteins indicative of altered synaptic signalling. Concurrently, adult hippocampal neurogenesis was decreased in middle age. Hippocampal microglia exhibited a lipid rich, inflammatory phenotype in middle age which correlated with poorer memory performance. CSF and serum proteomic and metabolomic analyses identified dysregulated lipid-related pathways potentially contributing to hippocampal vulnerability in middle age. Furthermore, 16S rRNA sequencing revealed reduced abundance of bacteria involved in lipid metabolism regulation. However, faecal microbiota transfer from young to middle aged rats was not sufficient to robustly improve hippocampus-dependent spatial memory. Together, these findings highlight dysfunctional lipid metabolism as a key feature of middle age that may contribute to decline in hippocampal function. Given that the scope for intervention is limited during older age, targeting biomarkers involved in metabolic and lipid homeostasis may be pivotal for the development of pharmacological or lifestyle-based interventions during middle age which could ultimately delay future cognitive ageing.

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Animals Hippocampus Gastrointestinal Microbiome Lipid Metabolism Aging Neurogenesis Male Microglia Proteomics Spatial Memory Memory CSF Gut microbiota Lipid metabolism Middle age Journal Article

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Maria Giovanna Caruso, Sebastian Dohm-Hansen, Francisco Donoso, Zoë A P Williams, Stefanie Grabrucker, Aonghus Lavelle, Jane A English, Sarah Nicolas, Olivia F O'Leary, Yvonne M Nolan. 2026-07-10. Lipid metabolism is a key central, systemic and gut microbial feature of the decline in rat hippocampal function during middle age.. https://doi.org/10.1016/j.neurobiolaging.2026.07.001

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Journal Article resource Aging subject Animals subject Gastrointestinal Microbiome subject Gut microbiota subject Journal Article subject Lipid Metabolism subject Male subject memory subject

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