Metabolomic differences in the Ophiura sarsii complex from the Yellow Sea Cold Water Mass and Bering Sea Cold Pool.

Jun Li; Min Li; Xiaomei Liao; Anning Mou; Ningxia Xu; Tonghui Zhang; Xuelei Zhang; Xiao Wang; Qinzeng Xu

doi:10.1016/j.cbd.2026.101930

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Metabolomic differences in the Ophiura sarsii complex from the Yellow Sea Cold Water Mass and Bering Sea Cold Pool.

Jun Li·Min Li·Xiaomei Liao·Anning Mou·Ningxia Xu·Tonghui Zhang·Xuelei Zhang·Xiao Wang·Qinzeng Xu

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Abstract

Metabolomics provides a functional readout of cellular physiology and can reveal metabolite-level differences associated with environmental and evolutionary contexts. Here, we used GC-MS- and LC-MS-based metabolomics to characterize metabolic profiles of the Ophiura sarsii complex from the Yellow Sea Cold Water Mass (YSCWM) and the Bering Sea Cold Pool (BSCP). This metabolomics analysis identified 398 LC-MS/MS and 87 GC-MS/MS differential metabolites (DEMs). Marked metabolic differences were observed between the two taxa, involving antioxidant-related metabolites, central carbon-related intermediates, osmolyte-associated compounds, and membrane lipid components. O. sarsii vadicola from the YSCWM showed higher levels of glutathione, glucose, citric acid, D-ribulose 5-phosphate, and unsaturated lipid-related metabolites, indicating differences in antioxidant-related and energy-associated metabolic profiles. By contrast, O. sarsii from the BSCP was characterized by higher levels of sugar alcohols, particularly myo-inositol, together with differences in membrane lipid-associated metabolites. These results provide metabolomics-based evidence for metabolite-level physiological differences between two members of the O. sarsii complex sampled from the Yellow Sea Cold Water Mass and the Bering Sea Cold Pool, while the relative contributions of lineage divergence and site-specific environmental variation remain to be tested experimentally.

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Metabolomics Metabolome Cold Temperature Seawater Gas Chromatography-Mass Spectrometry Antioxidant-related metabolites Cold-water benthos Membrane lipid remodeling Ophiuroidea Untargeted metabolomics Journal Article

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Jun Li, Min Li, Xiaomei Liao, Anning Mou, Ningxia Xu, Tonghui Zhang, Xuelei Zhang, Xiao Wang, Qinzeng Xu. 2026-07-07. Metabolomic differences in the Ophiura sarsii complex from the Yellow Sea Cold Water Mass and Bering Sea Cold Pool.. https://doi.org/10.1016/j.cbd.2026.101930

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Journal Article resource Antioxidant-related metabolites subject Cold Temperature subject Cold-water benthos subject Gas Chromatography-Mass Spectrometry subject Journal Article subject Membrane lipid remodeling subject Metabolome subject Metabolomics subject Ophiuroidea subject

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