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PubMed · 42263534

Generation of a STRAIGHT-IN Dual AAVS1 hiPSC line with orthogonal landing pads for versatile DNA payload integration.

Babet van der Vaart·Albert Blanch-Asensio·Niels Geijsen·Christine L Mummery·Christian Freund·Richard P Davis
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Abstract

The STRAIGHT-IN platform is designed for facile genomic integration of DNA payloads into human induced pluripotent stem cells (hiPSCs) that contain a pre-inserted landing pad (LP). Here, we expanded the versatility of STRAIGHT-IN by introducing an additional, orthogonal LP into the unmodified allele of the safe harbor locus AAVS1. Specifically, we targeted the hiPSC line LUMC0099iCTRL04_AAVS1-bxb-v2 (hPSCreg LUMCi004-A-1), which already carried one LP. The resulting STRAIGHT-IN AAVS1 Dual line can integrate two independent DNA payloads in parallel, expanding the applicability of the platform for complex genomic engineering applications.

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HumansInduced Pluripotent Stem CellsDNACell LineDependovirusJournal Article

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Babet van der Vaart, Albert Blanch-Asensio, Niels Geijsen, Christine L Mummery, Christian Freund, Richard P Davis. 2026-06-06. Generation of a STRAIGHT-IN Dual AAVS1 hiPSC line with orthogonal landing pads for versatile DNA payload integration.. https://doi.org/10.1016/j.scr.2026.104028

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