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PubMed · 42141726

CRISPR-mediated intronic knock-in of pre-amiRNA enables targeted gene silencing.

Jinsong Dong·Ke Zhang·Minglei Song·Menglan Xiao·Xianwei Lu·Shenghong Ge·Zai Zheng·Ming Li·Jian-Kang Zhu·Mingguang Lei
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Abstract

This study introduces an intronic artificial microRNA (IamiRNA) strategy that combines CRISPR-Cas9-mediated knock-in with endogenous miRNA processing for targeted gene silencing in plants. By inserting amiRNA precursors into introns of endogenous genes, this approach enables effective, tissue-specific gene silencing without persistent transgene expression, offering a promising tool for functional genomics and crop improvement.

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IntronsGene SilencingMicroRNAsPlants, Genetically ModifiedCRISPR-Cas SystemsGene Knock-In TechniquesJournal Article

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Jinsong Dong, Ke Zhang, Minglei Song, Menglan Xiao, Xianwei Lu, Shenghong Ge, Zai Zheng, Ming Li, Jian-Kang Zhu, Mingguang Lei. 2026-05-14. CRISPR-mediated intronic knock-in of pre-amiRNA enables targeted gene silencing.. https://doi.org/10.1016/j.xplc.2026.101916

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