Search PubMed⌕ Search
Home/Archive/PubMed

PubMed · 42098524

Biogenic Silver Nanoparticles from the Cell-Free Supernatant of Mychonastes sp. B1: Antibacterial and Antibiofilm Effects, and Wound Healing Activity Supported by Gene and Protein Expression Analysis.

Enver Ersoy Andeden·Serap Nigdelioglu Dolanbay·Gulsah Avci·Belma Aslim
View original source ↗Export metadata ↓

Abstract

The biogenic synthesis of silver nanoparticles (AgNPs) using microalgae provides a sustainable alternative to conventional physicochemical methods. In this study, AgNPs were synthesized from the cell-free supernatant of the freshwater microalga Mychonastes sp. B1 and characterized by ultraviolet-visible spectroscopy (UV-Vis), transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR), and field-emission scanning electron microscopy with energy-dispersive X-ray spectroscopy (FE-SEM/EDS). The nanoparticles were predominantly spherical (15-55&#xa0;nm), highly stable (&#x3b6;&#x2009;=&#x2009;&#x2009;-&#x2009;42.8&#xa0;mV), and appeared to be capped by extracellular polymeric substances. The biogenic AgNPs (GS-AgNPs) exhibited potent antibacterial activity, with minimum inhibitory concentrations (MICs) of 2.0&#xa0;&#xb5;g/mL against Staphylococcus aureus and 2.5&#xa0;&#xb5;g/mL against Pseudomonas aeruginosa, and significantly (p&#x2009;<&#x2009;0.05) inhibited biofilm formation. Fibroblast viability remained at or above 80% at AgNP concentrations up to 1.5&#xa0;&#xb5;g/mL, which promoted cell migration and increased wound closure by 8.1% at 24&#xa0;h (p&#x2009;<&#x2009;0.05). Exposure to 1.5&#xa0;&#xb5;g/mL AgNPs significantly upregulated extracellular matrix markers (Col1a1 2.3-fold, Fn1 3.3-fold at mRNA level; COL1A1 2.1-fold, FN1 2.7-fold at the protein level). These findings indicate that GS-AgNPs possess antimicrobial and wound healing properties, highlighting their potential as biocompatible nanomaterials for biomedical applications.

Explore related subjects

SilverMetal NanoparticlesAnti-Bacterial AgentsBiofilmsWound HealingMicrobial Sensitivity TestsStaphylococcus aureusPseudomonas aeruginosaChlorophytaMicroalgaeFibroblastsAntibacterial activityAntibiofilm effectGreen synthesisSilver nanoparticlesWound healingJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Enver Ersoy Andeden, Serap Nigdelioglu Dolanbay, Gulsah Avci, Belma Aslim. 2026-05-07. Biogenic Silver Nanoparticles from the Cell-Free Supernatant of Mychonastes sp. B1: Antibacterial and Antibiofilm Effects, and Wound Healing Activity Supported by Gene and Protein Expression Analysis.. https://doi.org/10.1007/s12033-026-01579-w

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2025

Biogenic Synthesis and Characterization of Hypecoum pendulum Mediated Silver Nanoparticles: Revealed Outstanding Anticancer and Genotoxic Potentials.

Fabrication of silver nanoparticles by green approach is the most effective and eco-friendly technique in recent technologies. The current study aimed to generate a simple, valid, and justifiable method for biogenic synthesis of silver nanoparticles (HP-AgNPs) using aqueous extract of Hypecoum pendulum L.(HP) and to assess their in&#xa0;vitro anticancer and genotoxic potentials on baby hamster kidney cell (BHK-21) and human blood lymphocytes using 3-(4,5-dimethylthiazol-2-yl-)-2,5-di-phenyltetrazolium bromide (MTT) and alkaline comet assay, respectively. HP-AgNP characterization was done using UV-vis spectrometry, EDX, SEM, XRD, and FTIR techniques. The crystalline nature of HP-AgNPs with a particle size of 36.3&#x2009;nm was assessed using the XRD technique. The surface morphologies with a particle size of 80&#x2009;nm were verified by SEM analysis. UV spectroscopy verified the existence of HP-AgNPs by yielding a sharp peak at 417&#x2009;nm with an absorbance intensity of 1.54. FTIR assessment revealed the existence of different functional moieties that contribute to the HP-AgNPs stabilization and reduction. Similarly, EDX analysis revealed Ag as a principal element (49%). MTT assay showed significant cytotoxicity by Doxorubicin and HP-AgNPs with a smaller IC50 value of 104.21&#x2009;&#xb1;&#x2009;4.33 and 134.91&#x2009;&#xb1;&#x2009;6.33&#x2009;&#x3bc;g/mL correlated to HP extract (229.84&#x2009;&#xb1;&#x2009;4.66&#x2009;&#x3bc;g/mL). The outcomes of the comet assay revealed potential DNA damage in a positive trend with concentration (25-600&#x2009;&#x3bc;g/mL). HP-AgNP-treated lymphocytes showed higher DNA damage as compared to HP extract-treated cells, but less damage as compared to a positive control, H2O2. These outcomes showed that HP-AgNPs have demonstrated promising anticancer and genotoxic action than HP extract due to their size and shape.

Silver↗