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PubMed · 41918234

Comparative Multiomics Analysis of Cerebral Organoid-Derived Exosomes during Organoid Maturation.

Young Eun Kim·Seong Soon Kim·Jangjae Lee·Sung Bum Park·Jeong Hyeon Jo·Hye Jin Cho·Kyoung Jin Choi·Jeonghui Im·Woo Rin Seo·Yeonji Park·Sunghwan Kim·Myung-Ae Bae·Ki Young Kim·Sung-Hee Cho·Dukjin Kang·Byumseok Koh
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Abstract

Cerebral organoids derived from human pluripotent stem cells recapitulate key features of early brain development and provide a physiologically relevant model for neurogenesis. Exosomes secreted by these organoids carry bioactive cargo and offer a noninvasive means to monitor maturation and intercellular communication. We performed comprehensive multiomics profiling of exosomes collected from cerebral organoids at defined developmental stages to evaluate their utility as biomarkers of neuronal differentiation. Metabolomic analysis revealed a progressive decline in amino acids, including glutamic acid, consistent with increased metabolic demand during neurogenesis. Lipidomic and neurosteroid profiling showed dynamic increases in phosphatidylethanolamine and pregnenolone, reflecting synaptic membrane formation and signaling. Transcriptomic and proteomic analyses identified stage-specific neurodevelopmental signatures, with key markers mirroring those of parent organoids. Collectively, cerebral organoid-derived exosomes faithfully reflect organoid maturation and provide a robust platform for tracking in vitro brain development.

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HumansOrganoidsExosomesMultiomicsBrainNeurogenesisProteomicsNeurodevelopmentCell DifferentiationMetabolomicsCerebral organoid-derived exosomeslipidomicsmultiomicsneurotransmittersproteomicstranscriptomicsJournal ArticleComparative StudyResearch Support, Non-U.S. Gov't

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Young Eun Kim, Seong Soon Kim, Jangjae Lee, Sung Bum Park, Jeong Hyeon Jo, Hye Jin Cho, Kyoung Jin Choi, Jeonghui Im, Woo Rin Seo, Yeonji Park, Sunghwan Kim, Myung-Ae Bae, Ki Young Kim, Sung-Hee Cho, Dukjin Kang, Byumseok Koh. 2026-03-31. Comparative Multiomics Analysis of Cerebral Organoid-Derived Exosomes during Organoid Maturation.. https://doi.org/10.1021/acs.nanolett.5c04673

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