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High NKAP expression predicts poor prognosis of breast cancer patients.

Yonghao Li·Zheng Shu·Chenyu Sun·Scott Lowe·Rachel Bentley·Yaru Li·Bethany King·Shuya Chen·Qin Zhou·Mei Zhang
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Abstract

NF-κB activating protein (NKAP) plays important roles in various cancers, including breast cancer. However, its expression and prognosis value in breast cancer remains uncertain. Gene expression profiling interactive analysis, Human protein atlas database, and University of Alabama at Birmingham Cancer data analysis portal database were used to predict the expression and prognostic value of NKAP in breast cancer. Immunohistochemistry, quantitative real time polymerase chain reaction (qRT-PCR) and western blot were performed to detect NKAP expression. The effects of NKAP on cell proliferation, migration and drug sensitivity were investigated in MDA-MB-231 and SK-BR-3 cells. NKAP protein expression differed in breast cancer tissues and paraneoplastic tissues based on the cancer genome atlas data. The high NKAP expression was significantly correlated with a poor prognosis in breast cancer patients. The results of immunohistochemistry, western blot assay, and qRT-PCR proved that NKAP was highly expressed in breast cancer tissues compared with paraneoplastic tissues. In addition, qRT-PCR results showed that high expression of NKAP was significantly correlated with the larger tumor size and higher TNM stage. Moreover, knockdown of NKAP significantly inhibited the proliferation, migration, and enhanced drug sensitivity of MDA-MB-231 and SK-BR-3 cells. NKAP is highly expressed in breast cancer tissues, and its high expression is closely associated with poor prognosis. NKAP also promotes proliferation, migration, and inhibits drug sensitivity of breast cancer cells.

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HumansFemaleBreast NeoplasmsPrognosisCell ProliferationCell MovementGene Expression Regulation, NeoplasticCell Line, TumorMiddle AgedMDA-MB-231 CellsNKAPbreast cancercell migrationcell proliferationdrug sensitivityJournal Article

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Yonghao Li, Zheng Shu, Chenyu Sun, Scott Lowe, Rachel Bentley, Yaru Li, Bethany King, Shuya Chen, Qin Zhou, Mei Zhang. 2026-02-06. High NKAP expression predicts poor prognosis of breast cancer patients.. https://doi.org/10.1097/md.0000000000047497

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