VANTAGE: van-based real-time HIV sequencing for transmission mapping and drug resistance profiling in war-affected Ukraine.

Ganna Kovalenko; Mariia G Liulchuk; Myroslava Filippovych; Pavlo Smyrnov; Steffanie A Strathdee; Tetyana I Vasylyeva

doi:10.1097/qad.0000000000004360

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VANTAGE: van-based real-time HIV sequencing for transmission mapping and drug resistance profiling in war-affected Ukraine.

Ganna Kovalenko·Mariia G Liulchuk·Myroslava Filippovych·Pavlo Smyrnov·Steffanie A Strathdee·Tetyana I Vasylyeva

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Abstract

We deployed the VANTAGE (VAN for Transmissible Agent Genomic Epidemiology) mobile system in Lviv, Ukraine, demonstrating end-to-end sequencing of dried blood spot samples within a clinic van usually serving de-occupied and frontline regions. HIV-1 pol sequences were obtained from 50% of samples, all subtype A6. Median time to 100× coverage was 38 min. Phylogenetic analysis revealed a local transmission cluster including a displaced person and the non-nucleoside reverse transcriptase inhibitor (NNRTI) resistance mutation E138A, supporting real-time HIV genomic surveillance in humanitarian crises.

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Humans Ukraine HIV Infections HIV-1 Drug Resistance, Viral pol Gene Products, Human Immunodeficiency Virus Sequence Analysis, DNA Genotype Molecular Epidemiology Phylogeny Mobile Health Units Male Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

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Ganna Kovalenko, Mariia G Liulchuk, Myroslava Filippovych, Pavlo Smyrnov, Steffanie A Strathdee, Tetyana I Vasylyeva. 2025-12-04. VANTAGE: van-based real-time HIV sequencing for transmission mapping and drug resistance profiling in war-affected Ukraine.. https://doi.org/10.1097/qad.0000000000004360

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