PubMed · 4118510
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M Green, D M Travis. 1973-01-20. Closing volume.. https://doi.org/10.1016/s0140-6736(73)90240-7
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The first 3He nuclear magnetic resonance (NMR) experiments using low-temperature prepolarization are presented. 3He cells were polarized at 4.2 K and 4.7 T, transported to another magnet, heated to room temperature, and used for NMR experiments at 2.35 T. Cells with and without a rubidium coating were tested. In both cases, the NMR signal was greater than 100 times the thermal equilibrium signal. No evidence of a rubidium coating effect on the longitudinal relaxation time T1 of 3He (500 mbar) at 4.2 K could be demonstrated. NMR gradient-echo images of the cells were acquired.
The structure of mitochondria was studied after the irradiation of human lymphocytes with an He-Ne laser (wavelength, 632.8 nm; dose, 56 J m-2). Ultrathin sections of the lymphocytes were studied by electron microscopy 1 h after the irradiation. The irradiation resulted in a 20% increase (p = 0.95) in the number of mitochondrial profiles on the cell section without an increase in their total area. Three-dimensional reconstruction of mitochondria from ultrathin sections trough the whole lymphocyte showed that the number of mitochondria was reduced to 9-12 in the irradiated cells compared with 40-45 in the control cells. In the irradiated lymphocytes, 2-4 giant branching mitochondria were also observed among small discrete mitochondria.