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Comprehensive analysis of DNA methylome and transcriptome reveals the epigenetic regulation of nitric oxide treatment in delaying apricot fruit senescence.

Yuanye Jiang·Jiejie Tao·Jinhua Zuo·Zixia Jing·Yunxiang Wang·Chunmei Bai·Alisdair R Fernie·Yanyan Zheng·Caie Wu
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Abstract

Apricot produces climacteric fruit, which are perishable after harvest. To elucidate the regulatory role of NO treatment through DNA methylation in post-harvest senescence, apricot fruits were treated with 0.2 mmol/L sodium nitroprusside (SNP) solution for 10 min, with distilled water treatment serving as the control. Treated fruits were then stored at 25°C and 80% relative humidity. Changes in appearance quality, physiological parameters, metabolome profiles, transcriptome dynamics, and DNA methylation patterns were analyzed before and after storage. Results showed that NO treatment delayed apricot softening, increased flavonoid metabolite accumulation, and reduced lipid and abscisic acid accumulation, with these effects correlated to the expression of specific genes and transcription factors. This work reveals the epigenetic regulatory mechanism underlying NO treatment delaying ripening and senescence. Further analysis revealed that the transcription levels of ACO, PAL, UFGT-like, NCED1, PP2C, MYB21, CCoAOMT-like, CYP707A, and ZNF7-like were all correlated with DNA methylation. This indicates that SNP treatment can lead to large changes in DNA methylation levels in apricot fruits, and that the differences in gene transcription levels are associated with the occurrence of hypomethylation and hypermethylation. Collectively, these findings establish an epigenetic framework for post-harvest regulation of apricot fruit, revealing DNA methylation-mediated freshness preservation mechanisms.

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DNA MethylationFruitEpigenesis, GeneticTranscriptomeGene Expression Regulation, PlantNitric OxidePrunus armeniacaPlant SenescenceEpigenomeDNA methylationepigeneticsmetabolomicstranscriptome analysisJournal ArticleResearch Support, Non-U.S. Gov't

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Yuanye Jiang, Jiejie Tao, Jinhua Zuo, Zixia Jing, Yunxiang Wang, Chunmei Bai, Alisdair R Fernie, Yanyan Zheng, Caie Wu. 2025. Comprehensive analysis of DNA methylome and transcriptome reveals the epigenetic regulation of nitric oxide treatment in delaying apricot fruit senescence.. https://doi.org/10.1111/tpj.70387

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