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PubMed · 40444400

Metagenomics or Metataxonomics: Best Practice Methods to Uncover the Sinus Microbiome.

Isabella Burdon·George Bouras·Kevin Fenix·Kenny Yeo·James Connell·Clare Cooksley·Emma Barry·Sarah Vreugde·Peter J Wormald·Alkis J Psaltis
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Abstract

Long-read-metagenomic sequencing is the best method for analyzing the sinus microbiome. 16s-rRNA-sequencing (both long and short read) results in PCR amplification bias that significantly distorts the sinus microbiome.

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HumansMetagenomicsMicrobiotaParanasal SinusesRNA, Ribosomal, 16SSinusitisbacteriologychronic rhinosinusitissinusitisGuidelineJournal Article

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Isabella Burdon, George Bouras, Kevin Fenix, Kenny Yeo, James Connell, Clare Cooksley, Emma Barry, Sarah Vreugde, Peter J Wormald, Alkis J Psaltis. 2025-05-30. Metagenomics or Metataxonomics: Best Practice Methods to Uncover the Sinus Microbiome.. https://doi.org/10.1002/alr.23617

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