Using Prime Editing Guide Generator (PEGG) for high-throughput generation of prime editing sensor libraries.

Samuel I Gould; Francisco J S&#xe1;nchez-Rivera

doi:10.1016/bs.mie.2025.01.006

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Using Prime Editing Guide Generator (PEGG) for high-throughput generation of prime editing sensor libraries.

Samuel I Gould·Francisco J Sánchez-Rivera

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Abstract

Prime editing enables the generation of nearly any small genetic variant. However, the process of prime editing guide RNA (pegRNA) design is challenging and requires automated computational design tools. We developed Prime Editing Guide Generator (PEGG), a fast, flexible, and user-friendly Python package that enables the rapid generation of pegRNA and pegRNA-sensor libraries. Here, we describe the installation and use of PEGG (https://pegg.readthedocs.io) to rapidly generate custom pegRNA-sensor libraries for use in high-throughput prime editing screens.

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Gene Editing RNA, Guide, CRISPR-Cas Systems Software Gene Library CRISPR-Cas Systems High-Throughput Screening Assays Humans Genome editing PegRNA Prime editing Python Journal Article

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BibTeX RIS

Samuel I Gould, Francisco J Sánchez-Rivera. 2025-03-06. Using Prime Editing Guide Generator (PEGG) for high-throughput generation of prime editing sensor libraries.. https://doi.org/10.1016/bs.mie.2025.01.006

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