Definition of the human mitochondrial TOM interactome reveals TRABD as a new interacting protein.

Metin &#xd6;zdemir; Silke Oeljeklaus; Alexander Schendzielorz; Marcel Morgenstern; Anusha Valpadashi; Roya Yousefi; Bettina Warscheid; Sven Dennerlein

doi:10.1242/jcs.263576

PubMed · 40105103

Definition of the human mitochondrial TOM interactome reveals TRABD as a new interacting protein.

Metin Özdemir·Silke Oeljeklaus·Alexander Schendzielorz·Marcel Morgenstern·Anusha Valpadashi·Roya Yousefi·Bettina Warscheid·Sven Dennerlein

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Abstract

The mitochondrial proteome arises from dual genetic origins. Nuclear-encoded proteins need to be transported across or inserted into two distinguished membranes, and the translocase of the outer mitochondrial membrane (TOM) complex represents the main translocase in the outer mitochondrial membrane. Its composition and regulation have been extensively investigated within yeast cells. However, we have little knowledge of the TOM complex composition within human cells. Here, we have defined the TOM interactome in a comprehensive manner using biochemical approaches to isolate the TOM complex in combination with quantitative mass spectrometry analyses. With these studies, we defined the pleiotropic nature of the human TOM complex, including new interactors, such as TRABD. Our studies provide a framework to understand the various biogenesis pathways that merge at the TOM complex within human cells.

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Humans Mitochondria Mitochondrial Precursor Protein Import Complex Proteins Mitochondrial Proteins Mitochondrial Membrane Transport Proteins Mitochondrial Membranes Protein Binding Mitochondrial biogenesis Mitochondrial protein import Mitochondrial quality control Protein transport TOM complex Journal Article Research Support, Non-U.S. Gov't

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Metin Özdemir, Silke Oeljeklaus, Alexander Schendzielorz, Marcel Morgenstern, Anusha Valpadashi, Roya Yousefi, Bettina Warscheid, Sven Dennerlein. 2025-04-24. Definition of the human mitochondrial TOM interactome reveals TRABD as a new interacting protein.. https://doi.org/10.1242/jcs.263576

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