Short-term diet intervention comprising of olive oil, vitamin D, and omega-3 fatty acids alters the small non-coding RNA (sncRNA) landscape of human sperm.

Candida Vaz; Mark Burton; Alexandra J Kermack; Pei Fang Tan; Jason Huan; Tessa P X Yoo; Kerry Donnelly; Susan J Wellstead; Dennis Wang; Helena L Fisk; Franchesca D Houghton; Sheena Lewis; Yap Seng Chong; Peter D Gluckman; Ying Cheong; Nicholas S Macklon; Philip C Calder; Anindya Dutta; Keith M Godfrey; Pankaj Kumar; Karen A Lillycrop; Neerja Karnani

doi:10.1038/s41598-024-83653-4

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Short-term diet intervention comprising of olive oil, vitamin D, and omega-3 fatty acids alters the small non-coding RNA (sncRNA) landscape of human sperm.

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Abstract

Offspring health outcomes are often linked with epigenetic alterations triggered by maternal nutrition and intrauterine environment. Strong experimental data also link paternal preconception nutrition with pathophysiology in the offspring, but the mechanism(s) routing effects of paternal exposures remain elusive. Animal experimental models have highlighted small non-coding RNAs (sncRNAs) as potential regulators of paternal effects. Here, we characterised the baseline sncRNA landscape of human sperm and the effect of a 6-week dietary intervention on their expression profile. This study involves sncRNAseq profiling, that was performed on a subset (n = 17) of the participants enrolled in the PREPARE trial: 9 from the control group and 8 from the intervention group. 5'tRFs, miRNAs and piRNAs were the most abundant sncRNA subtypes identified; their expression was associated with age, BMI, and sperm quality. Nutritional intervention with olive oil, vitamin D and omega-3 fatty acids altered expression of 3 tRFs, 15 miRNAs and 112 piRNAs, targeting genes involved in fatty acid metabolism and transposable elements in the sperm genome. PREPARE Trial registration number: ISRCTN50956936, Trial registration date: 10/02/2014.

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Humans Male Olive Oil Fatty Acids, Omega-3 Spermatozoa RNA, Small Untranslated Adult Vitamin D MicroRNAs Diet Diet intervention Small non-coding RNA Sperm miRNA piRNA tRF Journal Article

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Candida Vaz, Mark Burton, Alexandra J Kermack, Pei Fang Tan, Jason Huan, Tessa P X Yoo, Kerry Donnelly, Susan J Wellstead, Dennis Wang, Helena L Fisk, Franchesca D Houghton, Sheena Lewis, Yap Seng Chong, Peter D Gluckman, Ying Cheong, Nicholas S Macklon, Philip C Calder, Anindya Dutta, Keith M Godfrey, Pankaj Kumar, Karen A Lillycrop, Neerja Karnani. 2025-03-05. Short-term diet intervention comprising of olive oil, vitamin D, and omega-3 fatty acids alters the small non-coding RNA (sncRNA) landscape of human sperm.. https://doi.org/10.1038/s41598-024-83653-4

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