PubMed · 4002567
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W A Lester. 1985-04-13. Herdsman ability.. https://doi.org/10.1136/vr.116.15.423
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Species-specific monoclonal antibodies (MAbs) were developed against Mycoplasma agalactiae reference strain PG2 and French isolate P89 to study the in vitro expression of surface epitopes and to probe the antigenic profiles of 245 field isolates originating from 10 different countries. Colony immunostaining with MAbs on clonal lineage showed that 4 out of 9 species-specific epitopes exhibited a high rate of variation, demonstrating that M. agalactiae possesses a capacity for phenotypic diversification of its surface antigenicity. The emphasis was on dot immunobinding screening of the field isolates with MAbs recognizing permanently expressed epitopes. Eight different profiles could be defined. Great differences in epitope conservation were demonstrated with some area-specific strains completely lacking certain specific determinants. These results indicate that the antigenic variability of M. agalactiae relies not only upon surface switching mechanisms but also upon true epitope differences, partially related to the geographic origin of the isolates.
OBJECTIVE: To determine what effect participation by veterinarians in a dairy production medicine continuing education course would have on herd performance and management practices of client herds. DESIGN: Cohort study. SAMPLE POPULATION: 56 dairy herds for which health services were provided by veterinarians enrolled in a dairy production medicine continuing education program (treatment herds) and 97 dairy herds for which health services were provided by veterinarians not enrolled in the program (control herds). PROCEDURE: Management practices were evaluated every year for 4 years (1991 through 1994) by mail questionnaire. Herd performance was evaluated by reviewing Dairy Herd Improvement Association records. RESULTS: Mean age at first calving for the treatment herds decreased by 2 months over the course of the study. At the end of the study, treatment herds were 3 times more likely to review herd performance with their veterinarian and monitor heifer growth, and 2 times more likely to set goals and conduct adequate estrus detection than were control herds. CLINICAL IMPLICATIONS: Effective professional continuing education in production medicine can have a positive impact on health and performance of client herds.
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