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Glutamate molecular structure and protein affect the inhibition of breast cancer cell metastasis: Cell-derived exosomes inhibitory effects through the MAPK signaling pathway.

Yongcheng Chen·Huan Liu·Lang Zhang·Huifang Zeng·LiHe Jiang·Qinghong Qin·Dequan Li·Guanming Lu
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Abstract

The aim of this study was to investigate the inhibitory effect of glutamate molecular structure and protein on breast cancer cell metastasis and the potential inhibitory mechanism of cell-derived exosomes via MAPK signaling pathway. Breast cancer cell lines with high metastatic potential were selected by in vitro cell culture technique. The effects of specific inhibitors of glutamic acid on the proliferation and metastasis of breast cancer cells were studied. Changes in protein expression profiles were analyzed by proteomics techniques to identify key proteins associated with breast cancer metastasis. Breast cancer cells were treated with inhibitors of the MAPK signaling pathway to evaluate their effect on cell metastasis and compare with exosome treatment. The results showed that the specific inhibitors of glutamate molecular structure could significantly inhibit the proliferation and metastasis of breast cancer cells. Proteomic analysis revealed several down-regulated proteins that are closely related to breast cancer metastasis.

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HumansExosomesBreast NeoplasmsFemaleMAP Kinase Signaling SystemCell Line, TumorCell ProliferationGlutamic AcidNeoplasm MetastasisCell MovementProteomicsGene Expression Regulation, NeoplasticBreast cancerCell metastasisExosomeInhibition effectMAPK signaling pathwayMolecular structure of glutamateProteinJournal ArticleRetracted Publication

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Yongcheng Chen, Huan Liu, Lang Zhang, Huifang Zeng, LiHe Jiang, Qinghong Qin, Dequan Li, Guanming Lu. 2025-01-23. Glutamate molecular structure and protein affect the inhibition of breast cancer cell metastasis: Cell-derived exosomes inhibitory effects through the MAPK signaling pathway.. https://doi.org/10.1016/j.ijbiomac.2025.140264

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