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PubMed · 39699815

Quantitative Real-Time PCR for Circular RNA Detection and Analysis.

Amit Kumar Rai·Venkata Naga Srikanth Garikipati
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Abstract

In eukaryotes, nearly 2% of the genome represented by the coding proteins. However, emerging evidence suggest more than 75% of the human genome referred to as noncoding part also plays a crucial role in governing major regulatory pathways. Noncoding RNAs can be categorized into several groups, such as microRNAs (miRNAs), small nuclear RNA (snRNAs), small nucleolar RNA (snoRNAs), transfer RNA (tRNA), and circular RNA (circRNAs), which contribute to this regulatory landscape. Circular RNAs (circRNAs) are identified as a new class of regulatory noncoding RNAs with gene regulatory roles by acting as miRNA or RNA binding protein sponges or interacting with proteins. Researchers employ quantitative real-time PCR methods to examine circular RNA expression utilizing divergent primers for identification and quantification.

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RNA, CircularHumansReal-Time Polymerase Chain ReactionRNACircular RNADivergent primerRNase Rq-RT PCRJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov't

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Amit Kumar Rai, Venkata Naga Srikanth Garikipati. 2025. Quantitative Real-Time PCR for Circular RNA Detection and Analysis.. https://doi.org/10.1007/978-1-0716-4342-6_11

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