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PubMed · 39243857

ZASP: A Highly Compatible and Sensitive ZnCl2 Precipitation-Assisted Sample Preparation Method for Proteomic Analysis.

Xianfeng Shao·Yuanxuan Huang·Rong Xu·Qiqing He·Min Zhang·Fuchu He·Dongxue Wang
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Abstract

Universal sample preparation for proteomic analysis that enables unbiased protein manipulation, flexible reagent use, and low protein loss is required to ensure the highest sensitivity of downstream liquid chromatography-mass spectrometry (LC-MS) analysis. To address these needs, we developed a ZnCl2 precipitation-assisted sample preparation method (ZASP) that depletes harsh detergents and impurities in protein solutions prior to trypsin digestion via 10 min of ZnCl2 and methanol-induced protein precipitation at room temperature (RT). ZASP can remove trypsin digestion and LC-MS incompatible detergents such as SDS, Triton X-100, and urea at high concentrations in solution and unbiasedly recover proteins independent of the amount of protein input. We demonstrated the sensitivity and reproducibility of ZASP in an analysis of samples with 1 μg to 1000 μg of proteins. Compared to commonly used sample preparation methods such as SDC-based in-solution digestion, acetone precipitation, FASP, and SP3, ZASP has proven to be an efficient approach. Here, we present ZASP, a practical, robust, and cost-effective proteomic sample preparation method that can be applied to profile different types of samples.

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ProteomicsZinc CompoundsChloridesHumansChemical PrecipitationChromatography, LiquidTrypsinReproducibility of ResultsDetergentsLC-MSSDSTriton X-100ZASPincompatible detergentproteomicreproducibilitysample preparationsensitivityunbiasedJournal ArticleResearch Support, Non-U.S. Gov't

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Xianfeng Shao, Yuanxuan Huang, Rong Xu, Qiqing He, Min Zhang, Fuchu He, Dongxue Wang. 2024-09-06. ZASP: A Highly Compatible and Sensitive ZnCl2 Precipitation-Assisted Sample Preparation Method for Proteomic Analysis.. https://doi.org/10.1016/j.mcpro.2024.100837

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