Protocol to decode the role of transcriptionally active microbes in SARS-CoV-2-positive patients using an RNA-seq-based approach.

Aanchal Yadav; Priti Devi; Pallawi Kumari; Ranjeet Maurya; Uzma Shamim; Rajesh Pandey

doi:10.1016/j.xpro.2024.103071

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Protocol to decode the role of transcriptionally active microbes in SARS-CoV-2-positive patients using an RNA-seq-based approach.

Aanchal Yadav·Priti Devi·Pallawi Kumari·Ranjeet Maurya·Uzma Shamim·Rajesh Pandey

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Abstract

The elucidation of the role of microorganisms in human infections has been hindered by difficulties using conventional culture-based techniques. Here, we present a protocol for the investigation of transcriptionally active microbes (TAMs) using an RNA sequencing (RNA-seq)-based approach. We describe the steps for RNA isolation, viral genome sequencing, RNA-seq library preparation, and metatranscriptomic and transcriptomic analysis. This protocol permits a comprehensive evaluation of TAMs' contributions to the differential severity of infectious diseases, with a particular focus on diseases such as COVID-19. For complete details on the use and execution of this protocol, please refer to Devi et al.1.

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Humans SARS-CoV-2 COVID-19 RNA-Seq RNA, Viral Genome, Viral Sequence Analysis, RNA Transcription, Genetic Gene Expression Profiling Transcriptome Genomics Health Sciences Microbiology Journal Article Research Support, Non-U.S. Gov't

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Aanchal Yadav, Priti Devi, Pallawi Kumari, Ranjeet Maurya, Uzma Shamim, Rajesh Pandey. 2024-05-19. Protocol to decode the role of transcriptionally active microbes in SARS-CoV-2-positive patients using an RNA-seq-based approach.. https://doi.org/10.1016/j.xpro.2024.103071

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