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PubMed · 3838524

Algorithm for the correction of artifacts.

R C Zeimer·N P Blair·J G Cunha-Vaz
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Abstract

The sources of vitreous fluorophotometry artifacts are described. An algorithm to define this "spread function" is presented. The use of improved instrumentation and compensation for this artifact by data processing of the "correction" algorithm can improve the results of vitreous fluorophotometry. This algorithm was carried out in 40 eyes and was found to have an error of 0.7 mg/ml at 3 mm from the choroid-retina.

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ChoroidComputersFluoresceinFluoresceinsFluorescencePhotometryRetinaSoftwareJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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R C Zeimer, N P Blair, J G Cunha-Vaz. 1985. Algorithm for the correction of artifacts.. https://doi.org/10.1007/bf02133679

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Detection of histoplasma capsulatum DNA in lesions of chronic ocular histoplasmosis syndrome.

OBJECTIVE: To evaluate choroidal lesions in histological sections from the enucleated eye of a patient with chronic ocular histoplasmosis syndrome for the presence of Histoplasma capsulatum DNA. METHODS: Laser-capture microdissection was used to procure cells from macular and midperipheral choroidal lesions in a deparaffinized hematoxylin-eosin-stained section prepared from the enucleated left eye of a patient with an ipsilateral choroidal melanoma and bilateral chronic histoplasmosis syndrome. The captured cells were initially subjected to polymerase chain reaction (PCR) amplification using a pair of primers unique to each end of the nucleotide sequences that are complementary to the DNA known to flank the internal transcribed spacer regions of the ribosomal RNA genes of H capsulatum. This product was then reamplified using a second set of internally situated nested primers. The results were compared with a positive control sample of H capsulatum DNA and with a negative microdissected sample from noninflamed choroid in the same slide. RESULTS: Products of H capsulatum DNA were identified in both samples of microdissected tissue and the positive control. They were absent in the negative control. CONCLUSION: The observations provide molecular biological evidence linking the chronic choroidal lesions to earlier infection by H capsulatum.

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