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Differentiation-associated decrease in muscarinic receptor sensitivity in human neuroblastoma cells.

J E Heikkilä·I G Scott·L A Suominen·K E Akerman
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Abstract

Muscarinic receptor-linked increases in intracellular free Ca2+ as measured with quin-2 and Ca2+ release from monolayers of cells have been measured in the human neuroblastoma cell line SH-SY5Y. Induction of differentiation with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) leads to a decrease in the sensitivity of the cells to low concentrations of agonists with respect to the induced increase in cytosolic free Ca2+ and stimulation of Ca2+ efflux. No decrease in agonist binding affinity was observed when the displacement of a labelled antagonist, 3H-NMS, by a non-labelled agonist was studied.

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CalciumCell DifferentiationCell LineHumansKineticsMethacholine ChlorideMethacholine CompoundsN-MethylscopolamineNeuroblastomaReceptors, MuscarinicScopolamine DerivativesJournal ArticleResearch Support, Non-U.S. Gov't

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J E Heikkilä, I G Scott, L A Suominen, K E Akerman. 1987. Differentiation-associated decrease in muscarinic receptor sensitivity in human neuroblastoma cells.. https://doi.org/10.1002/jcp.1041300122

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