PubMed · 3471775
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R J Radlanski, A Jager. 1987. Vestibular shield.. https://pubmed.ncbi.nlm.nih.gov/3471775/
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Sedimentation field flow fractionation separation associated with flow cytometry has been used for the characterization of several commercial Saccharomyces cerevisiae yeasts used for wine production. A new type of channel 80 microm thick and new operating conditions, such as sample introduction when field and flow are established and a channel inlet connected to the accumulation wall, were used. Good repeatability (5% RSD) and reduced analysis time (2-10 min) were obtained. The avoidance of the stop-flow relaxation process in conjunction with the use of a channel of reduced thickness has demonstrated that an effective "steric-hyperlayer" mode driving to a major focusing effect of the species in the channel thickness is involved in the elution of the yeast cells. Flow cytometry analyses were performed, and the forward scattering and side scattering yeast characteristics correlation maps were obtained. Field flow fractionation and flow cytometry information obtained indicated that the fractogram profiles of the yeast cell depended not only on the size, but also on the shape and density.
The present paper reports significant enhancement of the detection power for total-reflection X-ray fluorescence (TXRF). The employment of an efficient wavelength-dispersive spectrometer rather than a conventional Si(Li) detector, as well as the use of a quasi-monochromatic undulator X-ray source, completely changed the quality of X-ray florescence spectra. The energy resolution is 20 times better, which effectively contributes to reducing the low-energy tail of the scattering background and to separating neighboring X-ray florescence peaks. Another advantage is its capability with respect to high-counting-rate measurements, which ensure the detection of weak signals from trace materials. The absolute and relative detection limit for nickel are 3.1 x 10(-16) g and 3.1 ppt (pg/g) for a 0.1-microL droplet of pure water, respectively, which is nearly 50 times better than the current best data achieved by conventional energy-dispersive TXRF using a Si(Li) detector system.