PubMed · 3456275
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R Brdicka. 1986-01-03. [Genetic engineering].. https://pubmed.ncbi.nlm.nih.gov/3456275/
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Reverse genetics of negative-sense RNA viruses, which enables one to generate virus entirely from cloned cDNA, has progressed rapidly over the past decade. However, despite the relative ease with which nonsegmented negative-sense RNA viruses can now be produced from plasmids, the ability to generate viruses with segmented genomes has lagged considerably, largely because of the inherent technical difficulties in providing all viral RNAs and proteins from cloned cDNA. A breakthrough in reverse genetics technology in the influenza virus field came in 1999, when we (Neumann et al., 1999, Proc. Natl. Acad. Sci. USA 96, 9345-9350) and others (Fodor et al., 1999, J. Virol. 73, 9679-9682) exploited a new approach to viral RNA production. In this review, we discuss the background for this advance, the systems that are now available for the generation of influenza viruses, and the implications of these developments for the future of virus research.
Ever since the initial discovery of the molecules and genes involved in disease resistance in plants, attempts have been made to engineer durable disease resistance in economically important crop plants. Unfortunately, many of these attempts have failed, owing to the complexity of disease-resistance signalling and the sheer diversity of infection mechanisms that different pathogens use. Although disease-resistant transgenic plants or seeds are not yet available commercially, future product development seems likely as our current level of understanding of pathogenesis and plant defence improves.
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